Concept

TGF-β is a key factor in balancing bone formation and synthesis.

Bone remodeling is the process through which the body removes old bone tissue from the skeleton and generates new bone tissue. Cells responsible for bone formation are called Osteoblasts, while cells responsible for breaking down bone tissue are known as Osteoclasts. High concentrations of TGF-β drive the differentiation of osteoblasts, promoting bone formation and synthesis.

Cell Biosci. 2013 Jan 15;3(1):4. doi: 10.1186/2045-3701-3-4.

ProbioFlex® Patent Data

ProbioFlex® Postbiotics used for experiments:
.Lactobacillus plantarum
.Bifidobacterium longum
Assessing the ability of chondrocytes
to secrete TGF-β.
Representing the ability to promote bone regeneration.
Details
Assessing the proliferative capacity of chondrocytes.
Representing the ability for bone repair.
Details

Assessing the ability of chondrocytes
to secrete TGF-β.

Experimental Method: Employing SW1353 Human Chondrocytes to Assess the Secretion of TGF-β.

In a 96-well culture plate, 200 μl of DMEM cell culture medium was added to each well.

SW1353 chondrocytes were seeded at a density of 1x104 cells/well in the 96-well culture. plate.

After 24 hours, the test substance was added.

Following an additional 24 hours, culture media were collected from different groups.

A commercially available TGF-β ELISA kit was employed to measure TGF-β concentration using the principle of antigen-antibody binding.

Before the start of the experiment, the issue of postbiotic aggregation was addressed. A technique known as “ProbioAid®” was employed to prevent and enhance the dispersion of prebiotics.

Many people believe that probiotics must be live to confer health benefits, but this is not necessarily the case. In recent years, there has been continuous research and scientific evidence demonstrating the health benefits and applications of heat-treated lactic acid bacteria (Heat treat LAB). Many products containing heat-treated bacteria have been introduced. To avoid confusion and the misconception that heat-treated bacteria are ineffective or considered 'dead,' the scientific community has coined a new term for them: 'Postbiotics.'
Postbiotics operate slightly differently from probiotics in producing health benefits. Postbiotics have a very small size, allowing them to directly enter the body through the intestinal mucosa. However, due to their small size, they are prone to clustering, significantly compromising their effectiveness. In order to address the issue of postbiotic aggregation, we have developed a technique over several years, and we are pleased to introduce 'ProbioAid®' – a technology designed to make postbiotics resistant to aggregation and enhance their performance.

ProbioAid® enhances the action-supporting effectiveness of postbiotics, resulting in a 122.6% improvement.

Postbiotics can help enhance mobility support, but when processed with ProbioAid® anti-aggregation and coating technology, the effects are significantly enhanced.

ProbioAid® increases the bone regeneration capability of prebiotics by more than 1-fold.

Various health ingredients possess varying degrees of bone regeneration capability!

Treating chondrocytes with low concentrations of health-care ingredients, it was found that ProbioAid® L.plantarum, ProbioAid® B.longum, hyaluronic acid and hydrolyzed type II collagen have strong TGF-β promoting capabilities.

"Postbiotics + Health ingredients"
The regenerative power of bone is further strengthened.

* p < 0.05 vs. health ingredients group
The synergistic effect of postbiotics combined with health ingredients significantly exceeds the efficacy of using health ingredients alone in promoting TGF-β production.

Assessing the proliferative capacity of chondrocytes.

Experimental Method: Employing SW1353 Human Chondrocytes to Assess the chondrocyte proliferation.

In a 96-well culture plate, 200 μl of DMEM cell culture medium was added to each well.
SW1353 chondrocytes were seeded at a density of 1x104 cells per well in a 96-well culture plate.
After 24 hours, the test substance was added.
After 24 hours, cell counting was performed under a microscope.

"Postbiotics + Health ingredients"
The cartilage repair capability is significantly enhanced.

* p < 0.05 vs. health ingredients group
The combination of postbiotics with health ingredients results in a synergistic effect on the cartilage cell repair capability, demonstrating a significant additive effect.
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